Detection of Cytochrome P450-2A6,-3A5 and 4B1 with Real-Time Polymerase Chain Reaction in Prostate Tissue

---

Atıf İçin Kopyala

Zencir S., ALPTEKİN D., Çeliktaş M., Canturk P., Çolak D., Caner V., ...Daha Fazla

ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES, cilt.63, ss.780-784, 2008 (SCI-Expanded)

• Yayın Türü: Makale / Tam Makale
• Cilt numarası: 63
• Basım Tarihi: 2008
• Doi Numarası: 10.1515/znc-2008-9-1026
• Dergi Adı: ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES
• Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
• Sayfa Sayıları: ss.780-784
• Çukurova Üniversitesi Adresli: Evet

Özet

Cytochrome P450 (CYP) is a heme-containing enzyme superfamily metabolizing a wide variety of xenobiotics, including drugs and carcinogens. The majority of CYP genes are expressed in the liver, however, some CYP isoforms are also reported for a number of extra hepatic tissues. We analyzed Cytochrome P450-2A6, -3A5 and -4B1 mRNAs using real-time reverse-transcriptase polymerase chain reaction (RT-PCR) in a total of 21 homogenized prostate tissue with or without malignancy. We detected a consistent expression of CYP2A6 and CYP3A5 in all, and of CYP4B1 in some (11/21) of the samples at mRNA level. Neither the histopathological status nor the smoking habit of the individuals affected CYP4B1 expression. Our results reflect possible roles for these particular CYPs in therapy and protection of prostate tissue.