Acta Horticulturae, cilt.1448, ss.113-120, 2026 (Scopus)
One of the most significant fruit crops in the world is citrus. Cross-pollination is still used in citrus breeding programs. Determination of pollen quality and quantity are important before cross-pollination for successful fertilization because poor pollen performance may adversely affect fruit set. In this study, in vitro pollen viability and pollen germination rates were determined in five different blood oranges (Citrus sinensis (L.) Osbeck), two different mandarins (Citrus clementina hort. ex Tanaka and Citrus reticulata Blanco), and kumquat (Fortunella margarita (Lour.) Swingle). The pollen quantities of these Citrus species were investigated before and after two-month storage. The percentage of pollen viability were tested with “TTC method”, and the pollen germination with “agar in petri method”. The maximum pollen viability and germination were recorded among the analysed species. In addition, pollen quantity of these cultivars was investigated. Since the amount of pollen in a flower of a cultivar was determined, it revealed the information about how much pollen should be stored in advance for pollination of citrus cultivars. The results of this study may be a pretty useful reference for storing pollen of species having different flowering periods.