Akademik Veri Yönetim Sistemi
Acta Horticulturae, cilt.1, sa.1439, ss.425-428, 2025 (Scopus)
Pistacia atlantica is naturally grown wild Pistacia species in Turkey and used as rootstock for pistachio. Due to long juvenile period of Pistacia species, rootstock breeding programs take quite long time with high cost. Therefore, there is necessity to perform early selection by sex determining of seedlings in the nurseries and to obtain the best rootstock seed sources after intra-and interspecific crosses in pistachio. The aim of this study is to detect variants in P. atlantica and to develop DNA markers linked to sex by QTL-seq mapping. A total of 25x clean data from female and male parents with bulks were obtained from ‘Pa1 x PaE’ intraspecies F1 population in P. atlantica. About 96 million reads belongs to bulks and 115 million reads for Pa1 female parent were determined as clean reads. These clean reads were aligned to P. atlantica reference genome, and QTL-seq software screed according to variants Delta SNP indexes for each mutation both bulks with 0.3 threshold. The candidate genomic regions within the first parts of chr14 and its 26-29 Mb fragments. A total of 49 SNPs and 36 InDels linked loci were detected for sex inheritance within the p<0.01** (p99) confidence interval. In conclusion, QTL-seq has significant power over the traditional mapping approaches for detection of the sex associated loci in P. atlantica. Therefore, they can be utilized for marker assisted selection at early stage in P. atlantica.