This study was conducted at the research area of Field Crops Department in Cukurova University, Agricultural Faculty in 2015 and 2016. The experimental was designed as a Randomized Completed Block design with three replications The objective of this study was to determinate the effect of seven different nitrogen fertilizer doses (0, 40, 80, 120, 160, 200 and 240 kg ha(-)1) and Rhizobium bacteria inoculation on some agronomic and quality characteristics of Halisbey peanut cultivar. In this study; the content of palmitic acid, stearic acid, oleic acid, linoleic acid, behenic and lignoceric acid of Halisbey peanut variety applied with different nitrogen doses were investigated. Fatty acids (palmitic acid, stearic acid, oleic acid, linoleic acid, behenic acid and lignoceric acid) content of Halisbey peanut variety was investigated. In the main cropped conditions, oleic acid was found very high in 24N+B (53.22%) and 20N+B (53.18%) applications. Linoleic acid gave the highest value in Control (30.39%) and 4N applications.