This study aimed to compare fatty acid and volatile compound compositions of Viburnum opulus fruits grown at various locations at different altitudes of Turkey (Ardahan/Center, Sivas/Gemerk, Kayseri/Develi, Gumushane/Kelkit). The total lipid contents in fruits varied from 9.34 % (Ardahan) to 12.35 % (Gumushane). A total of ten fatty acids (lauric acid (C12:0), myristic acid (C14:0), oleic acid (C 18:1), palmitic acid (C16:0), linoleic acid (C18:2), alpha-Linolenic acid(C18:3), arachidic acid (C20:0), gon doi c acid (C20:1), behenic acid (C22:0), stearic acid (C18:0)) have been identified and quantified. The major fatty acids in all the samples were oleic acid, linoleic acid, and palmitic acid. The highest saturated fatty acid (SFA) content was found in Gumushane sample (18.14 %), while the lowest content was detected in Sivas (13.46 %). Unlike SFA, the highest unsaturated faty acid (UFA) content was determined in Sivas sample (87.01 %), while the lowest content was identified in Gumushane sample (82.26 %). We used headspace and immersion solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME/GC-MS and Im-SPME/GC-MS) techniques to identify the volatiles. 23 and 35 compounds were identified by the HS-SPME/GC-MS technique at 28 and 40 degrees C, however, 44 and 38 compounds were detected by the Im-SPME/GC-MS technique at 28 and 40 degrees C. Thirty volatile components of V.opulus fruits have been detected for the first time in this study. 3-methylbutanoic acid in Ardahan, Kayseri, Sivas samples, ethyl acetate in Sivas sample, 2-octanol in Gumushane sample, phenol in Ardahan sample are the main volatile compounds. hn-SPME/GC-MS technique allowed identification of a larger number of volatile compounds and thus is more efficient than the HS-SPME/GC-MS technique.