MICROORGANISMS, cilt.8, sa.7, ss.1-14, 2020 (SCI-Expanded)
Oleaginous microorganisms, such as Yarrowia lipolytica, accumulate lipids that can have
interesting applications in food biotechnology or the synthesis of biodiesel. Y. lipolytica yeast can have
many advantages such as wide substrate range usage and robustness to extreme conditions, while
under several culture conditions it can produce high lipid productivity. Based on this assumption, in
this study, 12 different Yarrowia lipolytica strains were used to investigate microbial lipid production
using a glucose-based medium under nitrogen-limited conditions in shake-flask cultivations. Twelve
wild-type or mutant strains of Yarrowia lipolytica which were newly isolated or belonged to official
culture collections were tested, and moderate lipid quantities (up to 1.30 g/L) were produced; in
many instances, nitrogen limitation led to citric acid production in the medium. Lipids were mainly
composed of C16 and C18 fatty acids. Most of the fatty acids of the microbial lipid were unsaturated
and corresponded mainly to oleic, palmitic and linoleic acids. Linolenic acid (C18:3) was produced
in significant quantities (between 10% and 20%, wt/wt of dry cell weight (DCW)) by strains H917
and Po1dL.