The root lesion nematode, Pratylenchus thornei Sher & Allen, 1953 (Tylenchida: Pratylenchidae) are considered economically important plant parasitic nematodes affecting chickpea [Cicer arietinum L. (Fabales: Fabaceae)] production. A major strategy to develop resistance to root lesion nematodes in chickpea is to assess and exploit their natural variation. Therefore, nine accessions of wild Cicer reticulatum Ladiz. (Fabales: Fabaceae), Cicer echinospermum P.H.Davis (Fabales: Fabaceae) and domesticate C. arietinum were assessed for resistance to P. thornei according to multiplication rate of the nematode. This study was conducted during 2014-2015 to detect the suitable initial inoculum density (150, 225 and 300) per plant duration of experiment (16 and 20 weeks) for resistance test to P. thornei in chickpea cultivars. There was no significant difference between growing times 16 and 20 weeks and between the initial inoculum density of 225 and 300 nematodes. The only significant difference was observed at a low initial inoculum density of 150 nematodes in all tested cultivars. Therefore, the initial inoculum density of 225 and the growing time of 16 weeks were selected to access of chickpea genotypes for resistance study to P. thornei.