The effects of aging on the central nervous system steroid profiles and myelin basic protein in rats


Ciftci G., Yarim G. F., Yarim M., Karayigit M. Ö., Cenesiz S., Nisbet C., ...Daha Fazla

AGING CLINICAL AND EXPERIMENTAL RESEARCH, cilt.24, sa.2, ss.117-124, 2012 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 24 Sayı: 2
  • Basım Tarihi: 2012
  • Doi Numarası: 10.3275/7625
  • Dergi Adı: AGING CLINICAL AND EXPERIMENTAL RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.117-124
  • Çukurova Üniversitesi Adresli: Hayır

Özet

The aim of this study was to investigate the effects of aging on the central nervous system steroid and myelin basic protein (MBP) profiles. Forty-seven male Sprague-Dawley rats (newborn, 1, 6, 12 and 24-months-old) were studied. Tissues were obtained from the cerebellum and parietal, frontal, temporal cortex of the central nervous system of the rats for steroid extraction. The estradiol, progesteron, testosterone and dehydroepiandrosterone (DHEA) levels were measured by Enzyme Linked Immunosorbent Assay (ELISA). The average levels of estradiol (pg/g), progesteron (ng/g), DHEA (ng/g) and testosterone (ng/g) in the brain tissues were respectively 24.29, 4.59, 0.27, 0.92 in the newborn-rats; 4.18 +/- 1.10, 1.54 +/- 0.30, 0.28 +/- 0.01, 0.57 +/- 0.10 in the 1 month-old-rats; 11.02 +/- 1.10, 2.96 +/- 0.30, 0.27 +/- 0.01, 0.61 +/- 0.10 in the 6 month-old-rats; 15.80 +/- 1.10, 4.80 +/- 0.30, 0.28 +/- 0.10, 0.67 +/- 0.10 in the 12 month-old-rats; 20.07 +/- 1.10, 4.12 +/- 0.30, 0.28 +/- 0.01, 0.55 +/- 0.10 in the 24 month-old-rats. The myelin basic protein levels were determined by immunohistochemical staining and an elevation was observed in conjunction with the aging process. The results of the study indicate that the alterations in MBP, DHEA, progesterone, testosterone and estrodiol concentrations in the central nervous system of the rats during aging can be considered fundamental for future animal and human studies. (C) 2012, Editrice Kurtis