The study was conducted on ACLSV (Apple Chlorotic Leafspot Virus) isolates found in samples collected from temperate zone fruit orchards in Adana and Mersin provinces and districts in the Eastern Mediterranean region of Turkey. Reported as a natural host, Styrax officinalis bush was included in the study as a different host isolate (Kayseri). Leaf and cortical samples have been tested with commercial ELISA kits for the presence of common viruses. In order to verify DAS-ELISA results, the total RNAs were used in RT-PCR test and only positive responses were obtained from ACLSV-specific antiserum and primers. ACLSV isolates obtained from different hosts in the Eastern Mediterranean region were subjected to molecular variation analyses. Possible recombination sites of ACLSV were identified in the partial sequences of coat protein genes via comparison of ACLSV sequences from the GenBank database. As a result, for the first time recombinat or variant ACLSV isolates were reported in the region. Coat Protein Nucleotide sequences based on phylogenetic trees showed that these isolates were grouped and conserved signatures of type B6 clusters. This paper confirmed that Styrax officinalis as a natural ACLSV reservoir.