The utility of GFP in genetic engineering of horticultural plants

Yalcin-Mendi N. Y., Serbest-Kobaner S., Aka-Kacar Y.

EUROPEAN JOURNAL OF HORTICULTURAL SCIENCE, cilt.70, sa.4, ss.157-161, 2005 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 70 Sayı: 4
  • Basım Tarihi: 2005
  • Dergi Adı: EUROPEAN JOURNAL OF HORTICULTURAL SCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.157-161
  • Çukurova Üniversitesi Adresli: Evet

Özet

Reporter proteins play a significant role in developing and optimizing transformation protocols for plant species and they show a unique activity to visualize gene expression and protein localization. Chemical based selection for plant transformation is associated with a number of problems that might be avoided through visual selection. In recent years, the reporter protein GFP (Green Fluorescent Protein) has become very effective and highly valuable for use in biotechnology, cell biology, and biochemistry. It is a unique tool for monitoring gene expression, protein localization, detection of the gene flows and protein dynamics in both prokaryotic and eukaryotic living cells because it requires neither exogenous substrates nor cofactors for its activity. We discussed the use of jellyfish green fluorescent protein (GFP) as a reporter in horticultural plants.