Protein-coated microcrystals of Prunus armeniaca hydroxynitrile lyase: an effective and recyclable biocatalyst for synthesis of (R)-mandelonitrile


YILDIRIM D. , TOPRAK A. , ALAGÖZ D. , TÜKEL S. S.

CHEMICAL PAPERS, cilt.73, ss.185-193, 2019 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 73 Konu: 1
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1007/s11696-018-0577-5
  • Dergi Adı: CHEMICAL PAPERS
  • Sayfa Sayısı: ss.185-193

Özet

In this study, the crude solution of Prunus armeniaca hydroxynitrile lyase (ParsHNL) was simultaneously precipitated onto K2SO4 salt in acetone to immobilize ParsHNL as protein-coated microcrystals and the obtained preparations (PCMCs-ParsHNL) were used for the synthesis of (R)-mandelonitrile in buffer-saturated methyl tert-butyl ether (pH 4.0). The yield and enantiopurity of (R)-mandelonitrile were 100 and 90.5%, respectively, for free HNL, whereas the corresponding yield and enantiopurity values were 100 and 99.8%, respectively, for PCMCs-ParsHNL after 96h reaction time. The free HNL and PCMCs-ParsHNL were stored at room temperature and 5 degrees C during 30days and the results showed that PCMCs-ParsHNL had better storage stability compared to the free HNL at both room temperature and 5 degrees C. PCMCs-ParsHNL was imaged by a scanning electron microscopy and PCMCs-ParsHNL was rectangular in shape. PCMCs-ParsHNL was recycled five times in the synthesis of (R)-mandelonitrile and 75.2% initial activity recovery and 99.8% enantiomeric excess for (R)-mandelonitrile was determined after five uses. Thus, immobilizing ParsHNL as PCMCs provided highly active and reusable HNL preparations for (R)-mandelonitrile synthesis in buffer-saturated methyl tert-butyl ether.