Over-wintering of Penaeus semisulcatus (20.61 g) at three different temperatures (14, 18 and 22degreesC) was carried out in a greenhouse in three different closed recirculating systems. Temperature significantly influenced specific growth rate (SGR) and moulting interval (MI) (P<0.05). The SGR was 15-fold higher and the MI was 2.7-fold shorter at 22degreesC in comparison with 14degreesC. The relationship between SGR and temperature (T) was: Y=-0.053+0.070T (R-2=0.97). Shrimps over-wintered at 14degreesC consumed almost three times less food (0.43% body weight) than those at 22degreesC (1.28%) (P<0.05). A positive relationship (FR=0.426T-0.020, R-2=0.99) was observed between feeding rate (FR) and temperature. The survival rate achieved at 14, 18 and 22degreesC over the experimental period of 70 days was 87%, 67% and 73% respectively. Our data indicate that it is possible to over-winter this shrimp species in the sub-tropical regions by maintaining water temperature at around 14degreesC and 39 ppt salinity, in which little energy expenditure is needed, and the shrimp can survive and consume minimum amount of feed.