Turkey is one of the biggest cotton producing countries in the world. Defoliant is applied on 17% of the Turkey cotton production, which is similar to average defoliant application in the world. In Turkey, Finish (R) and Dropp (R) are widely in use as defoliant for desired boll opening in cotton. Finish (R) contains ethephon a.i. and cyclanilide a.i., and Dropp (R) contains thidiazuron a.i. and diuron a.i. In this study, total risk of defoliant on human health and environment were calculated by POCER (The Pesticide Occupational and Environmental Risk) indicator. The objective of this study was to determine the total risk value of defoliant application in cotton on human health and environment in Turkey. Thidiazuron a.i. has no potential risk on human health and environment. Diuron a.i. has relatively low total risk value on human health. Yet, it has very low risk on environment (0.097). Ethephon a.i. has relatively high total risk on human health (1.550). Cyclanilide a.i. has total risk on human health similar to ethephon a.i. These active ingredients (a.i.) have no potential risk for environment. Consequently, defoliant application in cotton has very low total risk value due to 10 of maximum value.