Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi, vol.17, pp.153-156, 2020 (National Refreed University Journal)
The purpose of this study was to determine the G>C mutation on exon 9 (G21234C) of Bovine Smoothened (SMO) gene polymorphism by using Cfr13I restriction enzyme with PCR-RFLP method in both Anatolian Black cattle breed and Anatolian Water Buffaloes. According to the G>C mutation on exon 9 (G21234C) of SMO gene, the results showed that there were three genotypes such as GG, GC and CC in Anatolian Black cattle, but there was only one genotype GG in Anatolian Water Buffaloes. Allele and genotype frequencies in Anatolian Black cattle were estimated as 0.35 and 0.65 for G and C alleles and 0.22, 026 and 0.52 for GG, GC and CC genotypes, respectively. The Chi-square test showed that the Anatolian Black cattle population was not in Hardy-Weinberg equilibrium with respect to G21234C substitution (P<0.05).
Because G>C mutation on exon 9 (G21234C) of SMO gene is polymorphic in cattle, it can be concluded that SMO gene is a potential candidate gene. However, such a statement cannot be expressed for buffaloes since it is monomorphic in Anatolian buffaloes.