The effect of a special adjuvant with FSH on blood FSH level, superstimulation and embryo quality in dairy cattle

Yilmaz M. A., Çökülgen T., Sevgi R., Okuroğlu A., Satllmlş M., SAY E., ...Daha Fazla

Journal of Dairy Research, cilt.92, sa.3, ss.404-411, 2025 (SCI-Expanded, Scopus)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 92 Sayı: 3
  • Basım Tarihi: 2025
  • Doi Numarası: 10.1017/s0022029925101246
  • Dergi Adı: Journal of Dairy Research
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Periodicals Index Online, Agricultural & Environmental Science Database, Analytical Abstracts, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Food Science & Technology Abstracts, Veterinary Science Database
  • Sayfa Sayıları: ss.404-411
  • Anahtar Kelimeler: cow, embryo quality, follicle-stimulating hormone, MontanideTM ISA-206 VG, superovulation
  • Çukurova Üniversitesi Adresli: Evet

Özet

Follicle-stimulating hormone (FSH) must be applied at 12-h intervals over 4-5 days in the traditional cattle superovulation protocol, which still needs to be improved. This research paper evaluated the superovulation results obtained by a traditional protocol or by a single administration of FSH dissolved in MontanideTM ISA-206 VG (MonISA-206). Control cows were superovulated with 10 mL of FSH (500 μg pFSH + 100 μg pLH) from day 7 to day 10 (for 4 days, twice daily i.m. injections, decreasing doses). Cows in the EG10 and EG7.5 groups were injected i.m. with 20 mL (100%, 10 mL + 10 mL) or 15 mL (75%, 7.5 mL + 7.5 mL) of the FSH and MonISA-206 mixture at once on day 7. All cows were inseminated 12 and 24 h after oestrus onset. The cows presented no pathology at the injection sites. Plasma FSH levels differed between the groups, but the interaction between hour and group × time was not different. Superstimulation and embryo quality results were similar between the groups. A single injection of FSH (both 100% and 75% doses) dissolved in MonISA-206 led to adequate plasma FSH levels and similar superovulation results to traditional FSH treatment, and caused no pathology at the injection sites.