In the present study, therapeutic effectiveness of dexamethasone, prednisolone and insulin were compared in ketotic dairy cows. Furthermore, the importance of some haematological and biochemical parameters in the diagnosis of ketosis was also evaluated. For this purpose, 21 holstein dairy cows with ketosis and 7 clinically healthy cows (control) were used. All the animals with ketosis divided into 3 groups equally. Each animal in first group received 750 mL of 30% serum dextrose Intravenouse (IV) and a dose of 4 mg/100 kg dexamethasone (IM) for 2 consecutive days. While, each animal in second group was injected with 750 mL of 30% serum dextrose Intravenouse (IV) plus a dose of 0.2 mg kg(-1) prednisolone Intramuscular (IM) daily for 2 days. Furthermore, 750 mL of 30% dextrose Intravenouse (IV) and 200 IU insulin were administered to the each animal in third group daily for 2 days and twice 48 h intervals, respectively. All the animals in Control Group were not received any treatment during the study. Urine samples and blood samples were collected from all the animals before and after treatment and then used to determine some haematological and biochemical parameters. It was concluded that ketosis causes significant alterations in some haematological and biochemical parameters in dairy cows. These parameters can be used for early diagnosis of ketosis in. dairy cattle, which may help to reduce the economical losses of dairy industry. Furthermore, the application of dextrose plus insulin was found to be more effective on the treatment of ketotic cows than other treatment methods applied. Therefore, the simultaneous use of dextrose and insulin can be recommended for the treatment of cows with ketosis.