The purpose of this prospective, double-blinded, and placebo-controlled clinical trial was to investigate the efficacy of topical cream, containing 0.1% retinoic acid for the treatment of tail gland hyperplasia in cats. Nineteen privately owned cats diagnosed with tail gland hyperplasia, based on history and clinical findings were randomly assigned to either a placebo or an active ingredient treatment group. Clinical evaluations were done by the same investigator, who scored clinical healing, at the beginning, during, and at the end of the treatment. Both owners and investigators were blinded to the allocation to the groups. It was demonstrated that retinoic acid gel treatment significantly decreased (P<0.05) the investigator's clinical scores while no significant changes were detected in the placebo treatment group. In conclusion, treatment with 0.1% retinoic acid cream was effective for the topical management of tail gland hyperplasia in the cats included in the study.