In this study, the frequencies of 111 antibiotic-resistant Gram-negative bacteria obtained from cultured sea bass were investigated. All the strains were identified and tested whether they were resistive against ten different antibiotics or not. The results showed that most of the bacteria were resistant to trimethoprim-sulphamethoxazole, cephalotin, tetracycline and streptomycin. It was found multiple antibiotic resistance (MAR) index values ranging from 0.3-0.8 for the bacteria isolated from gill. A large number of Pseudomanas putida, (25.2%), Moellerella wisconsensis (18%) and Pseudomonas fluorescens (10.81%) were identified. Also, strains of Ralstonia, pickettii (9%), Vibrio fluvialis (8.1%), Pantoea sp. (7.2%) and Erwinia sp. (5.4%) were found. This study suggested that Turkish sea bass farms have antibacterial multiresistance bacteria and may play as a reservoirs response for disease pathogen bacteria.