In the design of reinforced concrete structures, a designer must satisfy not only the strength requirements but also the serviceability requirements, and therefore the control of the deformation becomes more important. To ensure serviceability criterion, it is necessary to accurately predict the cracking and deflection of reinforced concrete structures under service loads. For accurate determination of the member deflections, cracked members in the reinforced concrete structures need to be identified and their effective flexural and shear rigidities determined. The effect of concrete cracking on the stiffness of a flexural member is largely dependent on both the magnitude and shape of the moment diagram, which is related to the type of applied loading. In the present study, the effects of the loading types and the reinforcement ratio on the flexural stiffness of beams has been investigated by using the computer program developed for the analysis of reinforced concrete frames with members in cracked state. In the program, the variation of the flexural stiffness of a cracked member has been obtained by using ACI, CEB and probability-based effective stiffness model. Shear deformation effect is also taken into account in the analysis and the variation of shear stiffness in the cracked regions of members has been considered by employing reduced shear stiffness model available in the literature. Comparisons of the different models for the effective moment of inertia have been made with the reinforced concrete test beams. The effect of shear deformation on the total deflection of reinforced concrete beams has also been investigated, and the contribution of shear deformation to the total deflection of beam have been theoretically obtained in the case of various loading case by using the developed computer program. The applicability of the proposed analytical procedure to the beams under different loading conditions has been tested by a comparison of the analytical and experimental results, and the analytical results have been found in good agreement with the test results. (C) 2009 Elsevier Ltd. All rights reserved.