First Report of Squash Mosaic Virus in Turkey

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Çağlar B. K., Güldür M. E., Yılmaz M. A.

Journal Of Plant Pathology, vol.86, no.2, pp.179, 2004 (SCI-Expanded)

  • Publication Type: Article / Abstract
  • Volume: 86 Issue: 2
  • Publication Date: 2004
  • Journal Name: Journal Of Plant Pathology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.179
  • Çukurova University Affiliated: Yes


Widespread symptoms of a possible virus disease were repeatedly observed in melon crops (Cucumis melo) grown in the provinces of Mersin, Adana, and Ur- fa (south-west Turkey). Affected plants showed mild to chlorotic mottling of the leaves, deformation of the blades, and chlorotic patches on the fruits. A virus was consistenly recovered from symptomatic leaf samples from different areas by mechanical transmission to herbaceous hosts. Symptoms in inoculated melon seedlings were the same as those observed in the field. ELISA tests of leaf extracts from naturally and artificial- ly infected melon plants gave positive reactions with two antisera to Squash mosaic virus (SqMV; genus Comovirus, family Comoviridae), supplied either by Dr. H. Lecoq (INRA, Montfavet, France) or a Turkish company (Lo- jistic). Since the seeds used to establish the crops had been imported from abroad and SqMV is known to be seed-borne, seeds from imported seed batches were ob- tained from the farms where the disease occurred. SqMV was consistently detected by ELISA in germinat- ed seeds from all batches. SqMV has several strains for three of which (Kimble, Arizona and Melon) partial se- quences are available (Hu et al., 1993; Haudenshield and Palukaitis, 1998). Strain-specific primers, designed by using Genbank sequences AF059533 (Kimble strain), AF059532 (Arizona strain), and M96148 (Melon strain), were used in PCR assays for amplifying reverse tran- scribed viral RNA extracted from diseased plants. Am- plicons of the expected size (579 bp) were obtained only with primers specific to the Kimble strain. This is the first report of the occurrence of SqMV in Turkey, where it has probably been introduced with imported seeds.