Akademik Veri Yönetim Sistemi
Journal of Tekirdag Agricultural Faculty, cilt.22, sa.2, ss.268-275, 2025 (ESCI)
Potato is an important source of food in developing countries, and potato viruses have been a major constraint on sustainable production of the potato crop in Sialkot, Punjab Province, Pakistan. During February 2021, a survey of nine commercial potato fields was conducted to determine the incidence of PVY, PVX, and PLRV, respectively. The results of serological and RT-PCR assays indicated that PVX was not detected in any of the fields. PVY and PLRV were dominant in all fields with infection levels up to 63.15% and 45.83% respectively. The incidence of co-infection of potato with PVY and PLRV was 8.3%. The RT-PCR was the most reliable method for the detection and identification of viruses. The coat protein sequences of PVY-SKT1, PVY-SKT2, PLRV-SKT1 and PLRVSKT2 were deposited in the Genbank database and assigned the accession numbers MW881191, MW881192 MW881193, and MW881194. The BLASTn search indicated PVY sequence shared the greatest similar score, at 99% similarity, with PVY isolates from China (HM036202.1) and Kashmir (KY851109.1). The PLRV sequence was most closely related to PLRV-Pakistan (MF276872.1) (99.8%) and China (KR051180.1) (99%) isolates. The results of co-inoculation experiments corroborated previous reports that infection of potato by multiple viruses dramatically increased disease severity (50-70%), compared to single virus infections (45-65%). The ELISA and RT-PCR results were confirmatory in that virus was detected in plants by serological and molecular methods, respectively. The prevalence of single and mixed PVY and PLRV infections in commercial potato fields underscores the need for routine virus indexing to foster routine planting of virus-free seed tubers.