In this study, immature zygotic embryo explants of 41 B American rootstock (V. vinifera L. cvs. 'Chasselas' x V. berlandieri) were cultured on B5 medium (Gamborg et al. 1968) supplemented with 0.5, 1.0, 2.0 and 4.0 mg l(-1) 2,4-D (2,4 dichlorophenoxy acetic acid) in light and dark conditions. The highest somatic embryo formation was obtained from dark condition in the media containing 0.5, 1.0 and 2.0 mg l(-1) 2,4-D with the rates of 27.5, 22.0 and 20.0 %, respectively. The highest number of torpedo shaped somatic embryos was also obtained from dark condition with B5 medium supplemented with 1.0 mg l(-1) 2,4-D (565 embryos per explant). These torpedo shaped somatic embryos were used to produce artificial seeds.