Citrus fruits are one of the most important fruit groups cultivated in the world. Growth and development of plants are affected by abiotic factors such as salinity and drought. Global climate change will increase water stress risk in the near future. One of the aims of plant biotechnology is the development of plants tolerant to drought. In the present study, the effect of in vitro drought stress on Carrizo citrange and expression levels of drought related genes were investigated. Four different PEG concentrations (1%, 2%, 4% and 6%) and PEG-free MS medium supplemented with 1 mg/L BA were used. The plants were subcultured 3 times at each 4 weeks. During each subculture period, the responses of plants to the drought were examined. Dry weight (gr), fresh weight (gr), shoot length (cm), and multiplication coefficient (unit / plant) were recorded. As a result of plant tissue culture studies, it was determined that plants continue to propagate in all media. However, there was a decrease in the multiplication coefficient in increasing PEG doses. In addition, expression levels of three different genes associated with drought in leaf samples from each subculture (SC) of plant were investigated and it was observed that genes had accelerating expression levels with increasing PEG doses. With these results, it has been revealed that drought-related genes are triggered.