This research was conducted to determine effect of pollinator bees on pod and seed setting of alfalfa clones throughout 2004-2007. The experiment was conducted as randomized split plot design with three replications under low land conditions. The eight alfalfa clonal lines, derived from different cultivars were used as experimental material. To determine effects of bee pollination, number of pods per raceme, seed number per pod, pod setting percentage and seed yield per plant were determined on both caged and open pollinated plants of the clonal lines. According to average of three years results, there were significant differences among alfalfa clonal lines in terms of above mentioned components. Under caged conditions, mean seed number per pod, pod number per raceme, pod setting percentage and seed yield per plant were determined as 1.3, 1.0, 5.9 and 0.8 g, respectively while under open pollination they were 8.1, 3.4, 47.6% and 12.7 g, respectively. The big differences in pod and seed setting could be attributed to the presence of bees. It was concluded that pollinator bees are essential in the seed production of alfalfa.