Immunohistochemical evaluation of akabane virus infection in aborted and new-born calves

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HALIGÜR M. , Hasircioglu S., OZMEN O., Kale M., AYDOĞAN A.

VETERINARNI MEDICINA, vol.59, no.5, pp.230-238, 2014 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 59 Issue: 5
  • Publication Date: 2014
  • Doi Number: 10.17221/7516-vetmed
  • Title of Journal : VETERINARNI MEDICINA
  • Page Numbers: pp.230-238


The present study was aimed at the detection and describing the lesions of akabane virus in foetal and new-born calves tissues using immunohistochemical and immunofluorescence techniques. Akabane virus lesions were evaluated in 12 foetuses and three new-born calves using serological and pathological methods and immunohistochemistry and immunofluorescence. Macroscopically, prominent arthrogryposis and hydranencephaly (A-H syndrome) were the main symptoms. At the histopathological examination, lesions were especially localised in the midbrain, pons, and medulla oblongata in the central nervous system (CNS) in calves naturally infected with akabane virus. In these areas, degenerative and necrotic neurons were observed. There was prominent mononuclear infiltration in perivascular areas. While akabane virus antigen was only detected in brain using immunofluorescence, immunohistochemistry against akabane virus yielded positive antigenic reactions in the cerebrum, cerebellum, liver, spleen and kidneys. In addition to these findings, there was a relationship between akabane virus infection and neurofilament (NF), glial fibrillary acidic protein (GFAP), and N-methyl-D-aspartate receptor (NMDAR) immunoreaction in astrocytes and neurons.