Large scale production of pepper somatic embryos using bioreactors


Buyukalaca S. , MAVITUNA F.

1st International Symposium on Solanacea for Fresh Market, Malaga, İspanya, 28 - 31 Mart 1995, ss.58-63 identifier

  • Cilt numarası:
  • Basıldığı Şehir: Malaga
  • Basıldığı Ülke: İspanya
  • Sayfa Sayısı: ss.58-63

Özet

Bioreactors can he used for large scale production of somatic embryos effectively as labour saving system. Two different types of bioreactor, an airlift and a magnetically stirred "hanging stirrer bar" bioreactor, each with 1.8 l working volume, and different mixing/aeration rates were examined to determine a system capable of in vitro pepper somatic embryo production. Production of pepper somatic embryos in a bioreactor was successful when a hanging stirrer bar bioreactor was used, Mixing, aeration, inoculum type and level affect directly the somatic embryogenesis in a bioreactor. The best results were obtained from hanging stirrer bar bioreactor at 0.45 vvm aeration and 80 rpm mixing rate in terms of percent embryogenesis. These results confirm that bioreactor culture offers promising aspects for large scale production.