Akademik Veri Yönetim Sistemi
6th INTERNATIONAL CONGRESS on APPLIED BIOLOGICAL SCIENCE, Adana, Türkiye, 8 - 09 Aralık 2021, ss.17
Nicotine, which is found as an alkaloid compound in the leaves of various plants, is a neuroteratogenic
substance and causes cognitive, emotional and behavioral anomalies in smokers. It is also a risk factor
for neurodevelopmental diseases. RBFOX3, TUBB3 and PAX6 genes have been shown to play a role
in the formation of neuron cells and neural differentiation during development. In our study, it was
aimed to investigate the possible effects of nicotine on neurons by focusing on the expression of these
genes.
SH-SY5Y cells differentiated into neurons by retinoic acid (10 µM). We divided cells into two groups:
nicotine and control. The cells in nicotine group were treated with 25ng/ml nicotine during
differentiation. After RNA isolation and cDNA synthesis, Real-time PCR reactions were performed for
RBFOX3, TUBB3 and PAX6 genes.
In our study, it was observed that RBFOX3, TUBB3 and PAX6 gene expressions were changed in the
nicotine group and there was a decrease in the gene expressions compared to the control group. As a
result, nicotine has a negative effect on neural differentiation during development by changing the
expression of the genes that have a role in neural differentiation.