Nicotine Decreases Expression of RBFOX3, TUBB3 and PAX6 Genes in SH-SY5Y Cells


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Pazarbaşı A., Uslu İ. N., Ay G.

6th INTERNATIONAL CONGRESS on APPLIED BIOLOGICAL SCIENCE, Adana, Turkey, 8 - 09 December 2021, pp.17

  • Publication Type: Conference Paper / Summary Text
  • City: Adana
  • Country: Turkey
  • Page Numbers: pp.17
  • Çukurova University Affiliated: Yes

Abstract

Nicotine, which is found as an alkaloid compound in the leaves of various plants, is a neuroteratogenic substance and causes cognitive, emotional and behavioral anomalies in smokers. It is also a risk factor for neurodevelopmental diseases. RBFOX3, TUBB3 and PAX6 genes have been shown to play a role in the formation of neuron cells and neural differentiation during development. In our study, it was aimed to investigate the possible effects of nicotine on neurons by focusing on the expression of these genes. SH-SY5Y cells differentiated into neurons by retinoic acid (10 µM). We divided cells into two groups: nicotine and control. The cells in nicotine group were treated with 25ng/ml nicotine during differentiation. After RNA isolation and cDNA synthesis, Real-time PCR reactions were performed for RBFOX3, TUBB3 and PAX6 genes. In our study, it was observed that RBFOX3, TUBB3 and PAX6 gene expressions were changed in the nicotine group and there was a decrease in the gene expressions compared to the control group. As a result, nicotine has a negative effect on neural differentiation during development by changing the expression of the genes that have a role in neural differentiation.