Variation in the high-molecular-weight (HMW) glutenin subunits of seed storage proteins of durum and common wheat may be useful for the improvement of pasta- and bread- making quality traits. A total of 945 protein samples from 91 Turkish landraces of Triticum turgidum var. durum were analyzed for their HMW glutenin subunits using SDS-PAGE. Nine different genotypes and a total of 12 HMW glutenin alleles (including null-alleles) were identified. In genome A (Glu-A1 locus) three alleles were found in Glu-A1-1 (encoding 'x-type' subunits) and one in Glu A1-2 (encoding'y-type' subunits), ranging in MW from 110 to 116 kD, At this locus, null alleles were detected in high frequency (63.5% in Glu-A1-1 and 100% in Glu-A1-2). The alleles at this locus formed three intragenomic combinations with only hire different band patterns. In genome B (Glu-B1 locus) four alleles were found in Glu-B1-1 (encoding 'x-type' subunits) and four in Glu-B1-2 (encoding 'y-type' subunits), ranging in MW from 85 to 104 kD, In contrast to the high frequency of null alleles in Glu-A1, no such alleles were detected in Glu-B1. The different subunits encoded by Glu-B1-1 and Glu-B1-2 genes formed six different intragenomic combinations. Hence, the three intragenomic allelic combinations of genome A (all subunits encoded by Glu-A1-1) and six of genome B gave rise to nine intergenornic combinations (i.e., nine genotypes), Comparing the HMW glutenin alleles of Central and Southeast Anatolia, we found that some alleles, such as Glu-A1- 1a, Glu-A1-1c, Glu-B1-1b, Glu-B1- 1c, Glu-B1-2b and Glu-B1-2b, were present in both regions, whereas Glu-A1-1b, Glu-B1-1a, Glu-B1-1d and Glu-B1-2c were restricted to Southeast Anatolia. The latter region exhibited a higher polymorphism than Central Anatolia.