DETERMINATION OF ANTIBIOTIC RESISTANCE AND CRISPR 2.1 LOCI OF ESCHERICHIA COLI ISOLATED FROM SEYHAN INLAND LAKE ADANA, TURKEY


Delik A., Yigittekin E. S. , USLU F. M. , DİNÇER S.

FRESENIUS ENVIRONMENTAL BULLETIN, cilt.29, ss.2784-2791, 2020 (SCI İndekslerine Giren Dergi) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 29
  • Basım Tarihi: 2020
  • Dergi Adı: FRESENIUS ENVIRONMENTAL BULLETIN
  • Sayfa Sayıları: ss.2784-2791

Özet

In this study we investigated relationship between existence of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) 2.1 locus and antibiotic resistance. The bacterial strains to be used in the experiment was isolated water samples taken from 5 different points of the Seyhan River. The antibiotic resistance tests of the isolated strains were performed by the disk diffusion method, the dendrogram analysis was calculated with UPGMA in the SHAN set and the MAR indices were specified. According to the MAR index, the highest and lowest antibiotic resistance was determined at respectively station 4 and 1. It is observed that none of the isolates developed resistance to Imipenem antibiotic. 147 of 150 isolates were identified as Escherichia coli (98%) with VITEK-2 Compact system. The presence of CRISPR 2.1 locus was identified in 53 of these 150 isolates (35.33%). The genome information was obtained with sequencing. it was determined as a result of the statistical analysis that there was no significant relationship between the MAR index values and spacer values (r= -0,098, p=0.50). In this study, no strong correlation was found between CRISPR 2.1 locus and multiple antibiotic resistance in environmental isolates of E. coli.