Immunomodulatory activities of pH/temperature sensitive smart naringenin-loaded nanopolymers on the mammalian macrophages

Yildirim M., Acet Ö.

Applied Surface Science Advances, cilt.18, 2023 (ESCI, Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 18
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1016/j.apsadv.2023.100527
  • Dergi Adı: Applied Surface Science Advances
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus
  • Anahtar Kelimeler: Naringenin, pH sensitive polymer, Raw 264.7 cells, Smart nanopolymers, Temperature sensitive polymer
  • Çukurova Üniversitesi Adresli: Hayır

Özet

This study aims to formulate naringenin (Nar)-loaded pH/temperature sensitive nanopolymers for therapeutic options for inflammatory diseases. For this aim, Nar loaded SNPs (NarSNPs) were synthesized by miniememulsion method and Scanning electron microscopy (SEM), Fourier-Transform Infrared Spectroscopy (FTIR) and, Dynamic Light Scattering (DLS) characterization experiments were conducted. The cytokines release (IL-6 and TNF-α) and cell viability were determined on lipopolysaccharide (LPS) induced RAW 264.7 macrophages. SNPs were determined as a potential naringenin carrier in terms of loading capacity, entrapment efficiency, pH/temperature dependent release, and preservation of bioactivity of Nar. According to our cell viability analyses, NarSNPs are safe at concentration 120 µg/mL. pH/temperature sensitive SNPs decreased the levels of the proinflammatory cytokines IL-6, TNF-α 3.95 and 4.35 times more efficiently than free Nar, respectively. These results indicate that SNPs increase bioactivity of Nar against adverse effects of inflammatory response.