Effect of neocuproine, a selective Cu(I) chelator, on nitrergic relaxations in the mouse corpus cavernosum


Gocmen C., Gokturk H. S., Ertug P., Onder S., Dikmen A., Baysal F.

EUROPEAN JOURNAL OF PHARMACOLOGY, vol.406, no.2, pp.293-300, 2000 (SCI-Expanded) identifier identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 406 Issue: 2
  • Publication Date: 2000
  • Doi Number: 10.1016/s0014-2999(00)00657-9
  • Journal Name: EUROPEAN JOURNAL OF PHARMACOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.293-300
  • Keywords: neocuproine, bathocuproine, corpus cavernosum, S-nitrosoglutathione, non-adrenergic non-cholinergic (NANC) nerve, nitrergic relaxation, RAT GASTRIC FUNDUS, S-NITROSOTHIOLS, NITRIC-OXIDE, SUPEROXIDE-DISMUTASE, SMOOTH-MUSCLE, NEUROTRANSMITTER, NITROSOGLUTATHIONE, ANOCOCCYGEUS, INHIBITION, NITROSOCYSTEINE
  • Çukurova University Affiliated: Yes

Abstract

The effects of neocuproine and bathocuproine, Cu(I) and Cu(II) chelators, respectively, were studied on relaxations in response to electrical field stimulation, acetylcholine, S-nitrosoglutathione, acidified sodium nitrite and sodium nitroprusside in the mouse corpus cavernosum precontracted with phenylephrine. Neocuproine significantly inhibited relaxations induced by electrical field stimulation, acetylcholine and S-nitrosoglutathione, but not by acidified sodium nitrite and sodium nitroprusside. The pre-prepared neocuproine-Cu(I) complex was ineffective on the responses. The discrepancy between the shape of relaxations in response to electrical field stimulation or to acetylcholine and S-nitrosoglutathione was abolished by adding CuCl2 into the bathing medium. The copper action was blocked by neocuproine but not by bathocuproine. However, the pre-prepared bathocuproine-Cu(II) complex did not accelerate the relaxations affected by CuCl2. These findings suggest that a Cu(I)-dependent mechanism may play a role in the relaxation induced by the endogenous relaxant factor as well as by S-nitrosoglutathione in mouse cavernosal tissue. (C) 2000 Elsevier Science B.V. All rights reserved.