Partial characterization of alpha-amylase enzymes produced by Bacillus isolates
Fresenius Environmental Bulletin, vol.31, no.02, pp.2259-2266, 2022 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 31 Issue: 02
- Publication Date: 2022
- Journal Name: Fresenius Environmental Bulletin
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aerospace Database, Agricultural & Environmental Science Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, Environment Index, Geobase, Greenfile, Metadex, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
- Page Numbers: pp.2259-2266
- Open Archive Collection: AVESIS Open Access Collection
- Çukurova University Affiliated: Yes
Abstract
In
this study, α-amylase producing Bacillus
strains ZF4 and A4 were isolated from the soil samples collected from Çukurova
University campus area. Bacillus sp.
ZF4 α-amylase showed its maximum activity at pH 6.0 and 40 °C, while Bacillus sp. A4 α-amylase at pH 8.0 and
40-50 °C. Moreover, ZF4 α-amylase retained all its activity after 15 minutes of
pre-incubation at 70 °C, but above this temperature, it lost its activity
rapidly. A4 α-amylase, on the other hand, maintained its activity up to 70 °C,
but experienced a rapid loss of activity at the temperatures above 80 °C. ZF4
α-amylase reached its highest activity level at 48 hours after inoculation,
while A4 α-amylase at 24 hours. While both enzymes reached higher activity
levels compared to the control group in the presence of SDS and Co; CaCl2,
MgCl2, and EDTA showed inhibitory effects on both enzymes. Finally,
in the zymogram analysis, it was found that both enzymes had a molecular weight
of approximately 105.4 kDa.