Partial characterization of alpha-amylase enzymes produced by Bacillus isolates

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Ozdolap M., Özcan B. D.

Fresenius Environmental Bulletin, vol.31, no.02, pp.2259-2266, 2022 (SCI-Expanded)

  • Publication Type: Article / Article
  • Volume: 31 Issue: 02
  • Publication Date: 2022
  • Journal Name: Fresenius Environmental Bulletin
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aerospace Database, Agricultural & Environmental Science Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, Environment Index, Geobase, Greenfile, Metadex, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Page Numbers: pp.2259-2266
  • Open Archive Collection: AVESIS Open Access Collection
  • Çukurova University Affiliated: Yes

Abstract

In this study, α-amylase producing Bacillus strains ZF4 and A4 were isolated from the soil samples collected from Çukurova University campus area. Bacillus sp. ZF4 α-amylase showed its maximum activity at pH 6.0 and 40 °C, while Bacillus sp. A4 α-amylase at pH 8.0 and 40-50 °C. Moreover, ZF4 α-amylase retained all its activity after 15 minutes of pre-incubation at 70 °C, but above this temperature, it lost its activity rapidly. A4 α-amylase, on the other hand, maintained its activity up to 70 °C, but experienced a rapid loss of activity at the temperatures above 80 °C. ZF4 α-amylase reached its highest activity level at 48 hours after inoculation, while A4 α-amylase at 24 hours. While both enzymes reached higher activity levels compared to the control group in the presence of SDS and Co; CaCl2, MgCl2, and EDTA showed inhibitory effects on both enzymes. Finally, in the zymogram analysis, it was found that both enzymes had a molecular weight of approximately 105.4 kDa.