Identification of Blastocystis hominis isolates from asymptomatic and symptomatic patients by PCR


Eroglu F., Genc A., Elgun G., KOLTAŞ İ. S.

PARASITOLOGY RESEARCH, vol.105, no.6, pp.1589-1592, 2009 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 105 Issue: 6
  • Publication Date: 2009
  • Doi Number: 10.1007/s00436-009-1595-6
  • Journal Name: PARASITOLOGY RESEARCH
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1589-1592
  • Çukurova University Affiliated: Yes

Abstract

Despite years of study, the pathogenic role of Blastocytis hominis is still controversial. Genotypic differences between the asymptomatic and symptomatic isolates should assist in determining the pathogenicity of Blastocystis. In this study, we genotyped 32 Blastocystis isolates obtained from 12 asymptomatic healthy individuals and 20 symptomatic patients pain by polymerase chain reaction using known seven kinds of sequence tagged site primers in this study. When we compared genotype of Blastocystis isolates between the symptomatic and asymptomatic patient group, we found that subtype3 is the most dominant genotype in asymptomatic individual (9/12) and subtype1 determined all of symptomatic patients (20/20).