Identification of Blastocystis hominis isolates from asymptomatic and symptomatic patients by PCR

Eroglu F., Genc A., Elgun G., KOLTAŞ İ. S.

PARASITOLOGY RESEARCH, cilt.105, sa.6, ss.1589-1592, 2009 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 105 Sayı: 6
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1007/s00436-009-1595-6
  • Dergi Adı: PARASITOLOGY RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1589-1592
  • Çukurova Üniversitesi Adresli: Evet

Özet

Despite years of study, the pathogenic role of Blastocytis hominis is still controversial. Genotypic differences between the asymptomatic and symptomatic isolates should assist in determining the pathogenicity of Blastocystis. In this study, we genotyped 32 Blastocystis isolates obtained from 12 asymptomatic healthy individuals and 20 symptomatic patients pain by polymerase chain reaction using known seven kinds of sequence tagged site primers in this study. When we compared genotype of Blastocystis isolates between the symptomatic and asymptomatic patient group, we found that subtype3 is the most dominant genotype in asymptomatic individual (9/12) and subtype1 determined all of symptomatic patients (20/20).