Effects of Pesticide Synergist Piperonyl Butoxide on Hepato-Oxidative Statues and Apototic Response in the Liver of Fenthion Exposed Oreochromis niloticus


Piner Benli P. , Üner N.

Fresenius Environmental Bulletin, vol.29, no.7, pp.5948-5956, 2020 (Journal Indexed in SCI)

  • Publication Type: Article / Article
  • Volume: 29 Issue: 7
  • Publication Date: 2020
  • Title of Journal : Fresenius Environmental Bulletin
  • Page Numbers: pp.5948-5956

Abstract

The aim of this study was to reveal the effects of piperonyl butoxide which is a pesticide synergist agent, modulator of cytochrome P450, on oxidative and apoptotic responses of fenthion, an organophosphate pesticide in the liver of Oreochromis niloticus selected as a model organism. For this purpose, 0.567 mg/L fenthion (¼ of LC50 value) and PBO concentration of 0.5 mg/L were applied for 24, 96 hours and 15 days. GPx, GR, GST and caspase enzyme activities; tGSH, GSH, GSSG, TBARS and HSP70 levels were analyzed in the liver.  The results show that PBO induced hepatotoxic effects of fenthion by altering stress proteins, lipid peroxidation and GSH metabolism in the liver of O. niloticus. Liver cells could not be protected by GSH-related antioxidant system from oxidative damage of these agents. Furthermore, the results also show that fenthion may have caused induction in apoptosis, and PBO might have increased this effect of fenthion by affecting caspase-3 enzyme activity depending on oxidative stress in the liver samples obtained from O. niloticus. We conclude that these types of synergistic agents may increase toxic effects of other pesticides including OP fenthion and may also cause concerns for human and animal health as well as  ecotoxicological effects.

The aim of this study was to reveal the effects of piperonyl butoxide which is a pesticide synergist agent, modulator of cytochrome P450, on oxidative and apoptotic responses of fenthion, an organophosphate pesticide in the liver of Oreochromis niloticus selected as a model organism. For this purpose, 0.567 mg/L fenthion (¼ of LC50 value) and PBO concentration of 0.5 mg/L were applied for 24, 96 hours and 15 days. GPx, GR, GST and caspase enzyme activities; tGSH, GSH, GSSG, TBARS and HSP70 levels were analyzed in the liver.  The results show that PBO induced hepatotoxic effects of fenthion by altering stress proteins, lipid peroxidation and GSH metabolism in the liver of O. niloticus. Liver cells could not be protected by GSH-related antioxidant system from oxidative damage of these agents. Furthermore, the results also show that fenthion may have caused induction in apoptosis, and PBO might have increased this effect of fenthion by affecting caspase-3 enzyme activity depending on oxidative stress in the liver samples obtained from O. niloticus. We conclude that these types of synergistic agents may increase toxic effects of other pesticides including OP fenthion and may also cause concerns for human and animal health as well as  ecotoxicological effects.