Akademik Veri Yönetim Sistemi
Journal of Turkish Phytopathology, cilt.51, sa.1, ss.21-26, 2022 (Hakemli Dergi)
Infected plant materials with Phytophthora citrophthora were collected from the outer and inner bark of lemon seedlings showing gummosis, crown and root rot in citrus orchard in Hatay province. Phytophthora isolates were obtained from infected five different lemon seedlings and purified on modified PARPH and PDA with different concentrations of antibiotics in vitro conditions. On the basis of colony morphology, five isolates were identified as P. citrophthora. The effect of culture media and temperature on mycelial growth of P. citrophthora were investigated. Colony patterns of P. citrophthora were performed on PDA, CMA, OMA, CA and HSA media and identified their microscopic structures. The radial mycelial growth of isolates was not dependent on media. In general, five different colony patterns of P. citrophthora were observed on the culture media; light and dense rosette pattern, slightly stellate pattern, stellate striated pattern, slightly petaloid pattern and non-pattern colony. The rate of the mycelial growth increased as temperature increased up to 25 °C and then decreased rapidly as temperature increased. Isolate Pc3 was determined the slowest while Pc1 and Pc5 were the fastest-growing isolates on agar media. The best mycelial growth of P. citrophthora was obtained on HAS medium while the least mycelial growth was obtained on CMA medium. Optimum temperature and the rate of mycelial growth were determined as 24.6 °C and 9.7 mm d-1 respectively.