the journal of child, ss.11-15, 2013 (Diğer Kurumların Hakemli Dergileri)
Objective: We examined the insertion (I) and deletion (D) polymorphism of angiotensin converting enzyme (ACE) gene polymorphism in 54 children with HSP to investigate the associated with presentation, prognosis and progression of children with Henoch-Schönlein purpura (HSP).
Materal and Methods:
Blood samples were collected from patients and obtained into EDTA. Genomic DNA from peripheral blood lymphocytes was purified. The ACE I/D gene polymorphism was detected by PCR with primer sequences derived.
The percent of ACE genotypes II, ID and DD in all patients was detected 4 %, 74 % and 22 % respectively. Patients were divided into 3 groups as HSP nephritis (n=14), HSP with minor urinary anomaly (n=18) and HSP without renal involvement (n=22). The distribution of ACE genotypes II, ID and DD respectively in HSP nephritis was 7 %, 57 %, 36 %; in HSP with minor urinary anomaly was 0 %, 89 %, 11 %; and in HSP without renal involvement was 5 %, 72 %, 23 % detected. No association was found between the ACE genotypes and the presence of renal involvement (χ2=4.39, p=0.356). The distribution of ACE allels I and D respectively in HSP nephritis was 36 % and 64 %; in HSP with minor urinary anomaly was 44 % and 56 % and in HSP without renal involvement was 41 % and 59 % detected. No association was found between the ACE allels and the presence of renal involvement (χ2=0.500, p=0.780).
In conclusion these results does not support a statistically significantly association between renal involvement and DD genotype or D allel in children with HSP. However D allele was detected in 96 % patients and DD genotype was higher in patients with HSP nephritis than patients without renal involvement. According these results we showed that D allel is a facilitative and I allel is a protective factor for development and renal involvement of disease. However larger studies are required to confirm these results.
ACE gene polymorphism, Henoch-Schönlein purpura