In the present study, school children of similar age-groups from two villages of Manisa province, namely Osmancali and Maldan, were examined in regular time periods to monitorize the changes in the incidence of head lice (Pediculus capitis). A total of 2091 children (1565 in Osmancali and 526 in Maldan) were combed with a newly-designed lice comb (PDC (R), KSL Consulting, Helsinge, Denmark) four times in May and November in 2008 and 2009. The assessment of the results was conducted with SPSS (R) v.15 program using CHAID and logistic regression analysis. The results of the periodic examinations of the children showed that the infestation rate of girls were found to be higher significantly compared to boys (P<0.001), and the incidence of head lice infestation in boys from Osmancali was found three times higher than the incidence in boys in Maldan (P=0.002). In addition, regular dry combing and treatment lowered the infestation rates among girls significantly (P=0.001). According to the results of the study, regular dry combing of school children with specially-designed lice combs may be essential for the control of this common infestation, especially in rural areas.