Acaricide resistance in Tetranychus urticae red form (Acari: Tetranychidae) collected from strawberry in southern Turkey: Bioassay and biochemical studies


Yalçın K., DÖKER İ., KAZAK C.

SYSTEMATIC AND APPLIED ACAROLOGY, vol.23, no.12, pp.2279-2287, 2018 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 23 Issue: 12
  • Publication Date: 2018
  • Doi Number: 10.11158/saa.23.12.1
  • Journal Name: SYSTEMATIC AND APPLIED ACAROLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.2279-2287
  • Keywords: Two-spotted spider mite, strawberry, acaricide resistance, bioassay, detoxification enzymes, 2-SPOTTED SPIDER-MITE, GLUTATHIONE-S-TRANSFERASE, PHYTOSEIULUS-PERSIMILIS, ABAMECTIN RESISTANCE, CINNABARINUS, IDENTIFICATION, POPULATIONS, MODE, INHERITANCE, TOXICITY
  • Çukurova University Affiliated: Yes

Abstract

Recent population outbreaks of Tetranychus urticae Koch (red form) (Acari: Tetranychidae) were observed in strawberry fields in southern Turkey. Growers frequently complained that acaricides becoming an inefficient means of controlling this polyphagous pest. Therefore, a laboratory based bioassay and biochemical tests were conducted to determine comparative acaricide resistance status of five fields and a lab population of T. urticae. Larval bioassays were conducted using etoxazole and spiromesifen, whereas adult bioassays were performed with abamectin and tebufenpyrad. Kinetic esterase and glutathione S-transferase (GST) enzyme activities were also determined using a microplate reader. When compared to the lab population, based on LC50 values, the resistance rations of field populations were found between 2.39-7.86, 6.80-15.39, 4.61-9.73, and 5.51-12.47-fold for abamectin, etoxazole, spiromesifen and tebufenpyrad, respectively. Total esterase and GST activities of field populations were much higher than of the lab population. A 7.72-10.69 mOD/min/mg protein and a 5.92-7.56 mOD/min/mg protein, esterase and GST enzyme activities were determined for field populations, respectively. Whereas these enzyme activities were found to be 3.83 and 5.49 mOD/min/mg protein for the susceptible lab population, respectively. The higher detoxification enzyme activities indicate that these two enzymes play an important role in metabolic pathway of acaricides in different T. urticae populations.