Method, medium and incubation time suitable for in vitro germination of eggplant (Solanum melongena L) pollen


Guler H., Abak K., Eti S.

1st International Symposium on Solanacea for Fresh Market, Malaga, İspanya, 28 - 31 Mart 1995, ss.99-105 identifier

  • Cilt numarası:
  • Basıldığı Şehir: Malaga
  • Basıldığı Ülke: İspanya
  • Sayfa Sayısı: ss.99-105

Özet

Suitable method, medium and incubation time for in vitro pollen germination of eggplant were investigated. "Agar in petri" method gave better results than "hanging drop" and "saturated petri" methods. The most convenient germination medium was 1% agar, 12% sucrose, 300 ppm H3BO3, and 300 ppm Ca(NO3)(2). Although percent germination was high in this medium, bursting of pollen grains and tubes was very frequent. Several incubation times were studied and 2-3 hours at 25 degrees C was found to be the most suitable time for counting the germinated pollen grains with no bursting. Suitability of the method, the medium, and the incubation time for evaluating pollen fertility was confirmed by comparing pollen germination results with pollen viability estimated by TTC-staining test in 11 eggplant F-1 hybrids.

Suitable methodmedium and incubation time for in vitro pollen germination of eggplant were investigated. "Agar in petri" method gave better results than "hanging drop" and "saturated petri" methods. The most convenient germination medium was 1% agar, 12% sucrose, 300 ppm H3BO3, and 300 ppm Ca(NO3)(2). Although percent germination was high in this medium, bursting of pollen grains and tubes was very frequent. Several incubation times were studied and 2-3 hours at 25 degrees C was found to be the most suitable time for counting the germinated pollen grains with no bursting. Suitability of the method, themedium,and the incubation time for evaluating pollen fertility was confirmed by comparing pollengermination results with pollen viability estimated by TTC-staining test in 11 eggplant F-1 hybrids.