Akademik Veri Yönetim Sistemi
Euphytica, cilt.222, sa.1, 2026 (SCI-Expanded, Scopus)
In this study, it was aimed to obtain haploid and then double haploid plants by ovary culture to accelerate the breeding studies in watermelon, which is consumed commonly in all over the world including Türkiye and has high economic value. For this purpose, twelve superior watermelon genotypes (Kar 17, Kar 22, Kar 37, Kar 48, Kar 68, Kar 88, Kar 116, Kar 165, Kar 180, Kar 192, Kar 340, Kar 341) selected from Cucurbit Genetic Resource Collection of Horticulture Department of Çukurova University and three commercial cultivars (Sturbust F1, Ersin F1 and Zümrüt F1) were used as plant material. In the experiments, a total of five MS-based nutrient media were tested: four for induction (A: Murashige and Skoog (MS), 30 g L⁻1 sucrose, 7 g L⁻1 agar, 0.04 mg L⁻1 TDZ; B: MS, 30 g L⁻1 sucrose, 7 g L⁻1 agar, 0.15 mg L⁻1 2,4-D, 1.5 mg L⁻1 kinetin; C: MS, 30 g L⁻1 sucrose, 7 g L⁻1 agar, 0.1 mg L⁻1 2,4-D, 1 mg L⁻1 BAP; D: MS, 30 g L⁻1 sucrose, 7 g L⁻1 agar, 0.1 mg L⁻1 2,4-D, 1 mg L⁻1 kinetin) and one for regeneration (MS supplemented with 0.2 mg L⁻1 BAP and 0.05 mg L⁻1 NAA). In the study, pre-treatments [high temperature (35 °C for 48 h), cold shock (4 °C for 24 h) pre-treatments and no pre-treatment] were applied to the ovaria and their effects on development were examined. In vitro and in vivo colchicine were applied to the plants determined to be haploid as a result of flow cytometry analysis. Molecular analyses were performed on the plants determined to be double haploid and their homozygosity was examined. As a result of the study, it was determined that the most effective induction medium was C and the most effectivepre-treatment was keeping the ovaries at 35 °C for 48 h. Out of 100 plants subjected to flow cytometry analysis, 67 plants were determined to be haploid and 33 plants were determined to be double haploid. The most successful colchicine application was determined to be keeping in 0.5% colchicine solution for 4 h with a success rate of 63.01%. The number of plants obtained with ovary culture and acclimatizated outside conditions was 294 and a total of 34 lines were obtained.