Flow-dependent drug response of breast Cancer organoids in an organ-on-Chip platform
Microchemical Journal, cilt.229, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 229
- Basım Tarihi: 2026
- Doi Numarası: 10.1016/j.microc.2026.119414
- Dergi Adı: Microchemical Journal
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Chemical Abstracts Core, Chimica, Index Islamicus, Academic Search Ultimate (EBSCO), Engineering Source (EBSCO)
- Anahtar Kelimeler: Breast cancer, Drug testing, MCF-7, Microfluidics, Organ-on-chip, Organoid
- Çukurova Üniversitesi Adresli: Evet
Özet
Breast cancer is a widely seen malignancy affecting women globally. Organoids, which are commonly used in breast cancer research, can replicate organ complexity. Therefore, they offer advantages for studying the nature of the disease and drug screening. Organ-on-Chip (OoC) devices can mimic organ structures and functions. With an interdisciplinary approach, the present study introduces an OoC device and evaluates the impact of ribociclib on MCF-7 organoids under various flow conditions. MCF-7-derived organoids were cultured in a microfluidic chip, and drug response tests were conducted under various flow rates (1, 5, and 10 μL/min). The study involves designing an OoC platform, manufacturing its molds with 3D printing, and fabricating the microchip using PDMS. The OoC device design was optimized through numerical simulations, which provide insights into flow dynamics and ribociclib transport within the device. MCF-7-derived organoids preserved their multicellular structure inside the device. Morphological analysis showed consistent organoid growth over the 15-day culture period. In the histology analysis, preserved structural features were observed. Moreover, active proliferation within the 3D structure was detected from Ki-67 staining. The magnitude of drug flow rate of 1, 5, and 10 μL/min has considerable effects on organoid viability and proliferation. This indicates that the OoC device can be used to assess drug responses under various flow conditions. The OoC platform successfully supported the culture of breast cancer organoids and enabled the evaluation of drug responses under dynamic microenvironments. The results demonstrate that the proposed OoC system has significant potential for drug testing in cancer research.