Vertebral morphometric studies provide information for both the diagnosis of spinal canal deformity and the design of spinal instrumentations and anthropometric models. This study sought to gather computed tomography-based morphometric data of the L3-L5 vertebrae in healthy adults. The computed tomography scans of 103 normal individuals (47 males and 56 females) of 20 to 51 years of age were studied to provide quantitative anatomic analyses on lumbar vertebrae at the L3-L5 level. In each lumbar vertebrae, the anterior and posterior heights of the vertebral body and disc, the height and width of the pedicle, and the diameters of the spinal canal were measured. Comparisons were made and associations were investigated. Significant differences were found between males and females for the mean dimensions of the vertebral bodies and for the disc heights (0.001 < P < 0.05). Disc heights also showed a significant decrease in a craniocaudal sequence in both sex (P < 0.000). The mean depth and width of the lumbar spinal canal increased progressively from L3 to L5 vertebrae (P < 0.000). Significant positive relationships were found between the upper and lower end-plate measurements (0.683 < r < 0.852). These morphometric data may provide useful information for the calculation of the anthropometric indices, which meet the changing clinical and surgical demands.