Investigators in this study explored levels of soluble CD27 (sCD27), interleukin (IL)-8, and IL-10 in B-cell chronic lymphocytic leukemia (B-CLL), and the correlation of these levels with disease stage and prognosis. Plasma IL-8, IL-10, and sCD27 levels were assessed with enzyme-linked immunosorbent assay tests in 22 healthy donors and 70 patients with B-CLL (49 men and 21 women). Mean patient age was 61.57 y (range, 44-75 y). Mean healthy donor age was 62.09 y (range, 40-72 y). in the study group, mean values were as follows: plasma L-8, 284.758 pg/mL (0-1000 pg/mL); plasma IL-10, 26.152 pg/mL (0-100 pg/mL); sCD27, 731.357 U/mL (139.9-1000 U/mL); white blood cell count, 59.9 x 10(9)/L (0.8-250.0 x 10(9)/L); hemoglobin count, 11.2 g/dL (5.0-16.2 g/dL); platelet count, 162.5 x 10(9)/L (2 9.8-317 x 10(9)/L); 132 microglobulin (B2M) 3350.2 mg/L (274.7-7499.9 mg/L); CD38, 19.50%; and lactate dehydrogenase (count, 497.5 U/L (263.0-1507 U/L). Patients represented all Rai stages, with 22.9%, at stage 0, 11.4% at stage 1, 11.4% at stage 11, 41.4%, at stage 111, and 12.9% at stage IV. Plasma levels of IL-8, IL-10, and sCD27 were correlated between study and control groups; significantly higher IL-8 (P=.001) and sCD27 (P=.000) levels were found, but the IL-10 level was not significant (P=.l 39). Plasma IL-10 (P=.01) and sCD27 (P=.008) were positively correlated with Rai stage, but IL-8 was not (P=. 146). Levels of sCD27 were significantly correlated with values for 132 M (P=.000), hemoglobin (P=.028), lactate dehydrogenase (P=.001), CD19 (P=.03), and IL-10 (P=.000). IL-8 was significantly correlated with white blood cell (P=.000) count, and CD38 (P=.001) and CD5 (P=.006) levels. IL-10 was significantly correlated with B-2,M (P=.01 7), CD1 9 (P=.000), platelet (P=.002), and CD27 (P=.000). In survival distributions for CD27, IL-8 and IL-10 were found to have more significant relationships for all parameters (P=.0000). In conclusion, the authors suggest that sCD27, IL-8, and IL-10 are more significant prognostic factors for B-CLL when compared with others, and these values should correlate with new prognostic factors (eg, zeta-associated protein-70, mutated/unmutated immunoglobulin variable heavy chain).