Importance of 14-3-3eta, anti-carp, and anti-sa in the diagnosis of seronegative rheumatoid arthritis


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Salman E. , Çetiner S. , Boral B. , Kibar F. , Erken E. , Ersözlü E. D. , ...More

Turkish Journal of Medical Sciences, vol.49, no.5, pp.1498-1502, 2019 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 49 Issue: 5
  • Publication Date: 2019
  • Doi Number: 10.3906/sag-1812-137
  • Title of Journal : Turkish Journal of Medical Sciences
  • Page Numbers: pp.1498-1502
  • Keywords: 14-3-3eta, Anti-carP, Anti Sa, seronegative, CYCLIC CITRULLINATED PEPTIDE, RHEUMATOLOGY/EUROPEAN LEAGUE, CLASSIFICATION CRITERIA, AMERICAN-COLLEGE, ANTIBODIES, ASSOCIATION, DISEASE

Abstract

Background/aim: Rheumatoid arthritis (RA) is an autoimmune disease characterized by synovial inflammation. The study aimed to

assess serum 14-3-3eta, anti-CarP, and anti-Sa in seronegative RA (SNRA) patients who were treatment-naïve as well as in healthy

subjects. This is the first study in the literature to examine these autoantibodies together in SNRA patients.

Materials and methods: Forty-five treatment-naïve SNRA patients and 45 healthy subjects were recruited. Drugs change the levels of

autoantibodies; therefore, patients who took any medication had been excluded from our study. Anti-carbamylated protein, anti-Sa, and

14-3-3eta were measured by using three different ELISA kits.

Results: Median serum concentration of healthy controls in 14-3-3eta was 0.02 (0.02–0.27) ng/mL. Median serum concentration of

SNRA patients in 14-3-3eta was 1.00 (0.48–1.28) ng/mL. Data were analyzed with Mann–Whitney U tests; the P-value was <0.001 in

14-3-3eta. Receiver operating characteristic (ROC) curve analysis showed that 14-3-3eta in SNR compared to healthy controls had a

significant (P < 0.001) area under the curve (AUC) of 0.90 (95% confidence interval, 0.83–0.96). At a cutoff of ≥0.33 ng/mL, the ROC

curve yielded a sensitivity of 88.9%, a specificity of 82.2%, a positive predictive value of 83.3%, and a negative predictive value of 88.1%.

Conclusion: We found that 14-3-3eta can be used as a diagnostic marker in SNRA.

Key words: 14-3-3eta, Anti-carP, Anti Sa, seronegative