The activity and kinetics of pyruvate kinase in hypoxic newborns


Narli N. , Satar M. , Kayrin L. , Yapicioglu H. , Ozlu F. , BUDGAYCI R.

PEDIATRIC HEMATOLOGY AND ONCOLOGY, cilt.22, sa.7, ss.567-573, 2005 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 22 Konu: 7
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1080/08880010500198616
  • Dergi Adı: PEDIATRIC HEMATOLOGY AND ONCOLOGY
  • Sayfa Sayıları: ss.567-573

Özet

Pyruvate kinase (PK) plays a key role in erythrocytes, which obtain most of their energy from glycolysis. This study investigated erythrocyte energy metabolism in hypoxic newborns, measuring pyruvate kinase activity, kinetic, and ATP levels in hypoxia. Forty-nine babies who had cord pH value lower than 7.2 and Apgar scores lower than 7 in the first minute were accepted as the hypoxic group, and 48 babies who had cord pH value higher than 7.2 and an Apgar score higher than 7 in the first minute were taken as controls. The erythrocyte mean PK activity was found to be lower (16.9 +/- 8.5 [5.8-47.91 EU/gHb) in the hypoxic group than the control group (21.3 +/- 10.9 [3.9-44.3] EU/gHb) (p <.05). The mean ATP value of hypoxic group was higher (19.2 +/- 11.3 [3.9-37.6] mM) compared to control group (13.8 +/- 7.16 [3.9-28.7] mM). In the kinetic study, with different ADP concentrations in the control group, the substrate amount (K-m) that is needed to reach the half-maximum of enzyme activity (V-max = 27.7 Eu/gHb) was found to be 2.70 mM, but it was 1.47 mM to reach V-max (22.7 Eu/gHb) in the hypoxic group. V-max was 41.67 Eu/gHb and K-m was 8.33 mM in the control group at different PEP concentrations, whereas V-max was 21.7 Eu/gHb and K-m was 0.89 mM in the hypoxic group. Increase in the ATP level while ePK activity decreases, suggesting that glycolysis increases in hypoxia. In the kinetic study, the substrate amount needed for reaching the half-maximum of enzyme activity was less in the hypoxic group, probably suggests that pyruvate kinase increases glycolysis by increasing its affinity to the substrates. In this way, erythrocytes may gain the energy required for oxygen delivery to tissues and maintaining ion gradient. This arrangement possibly proceeds from sygmoidal structure of pyruvate kinase.