Effect of auxin type and concentrations in different media on the callus induction and shoot formation of crested wheatgrass (Agropyron cristatum (L.) Gaertn)

Can E., Celiktas N., HATİPOĞLU R.

BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, vol.22, no.3, pp.782-786, 2008 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 22 Issue: 3
  • Publication Date: 2008
  • Doi Number: 10.1080/13102818.2008.10817553
  • Journal Name: BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.782-786
  • Keywords: crested wheatgrass, young inflorescences, in vitro culture, auxin, medium, CULTURED IMMATURE INFLORESCENCES, SOMATIC EMBRYOGENESIS, PLANT-REGENERATION, TISSUE-CULTURE, BLUEGRASS, DACTYLIS, EMBRYOS, GROWTH, PHLEUM, LOLIUM
  • Çukurova University Affiliated: Yes

Abstract

Effects of different medium (LS and SH).supplemented with various auxin concentrations (2, 4, 6 and 8 mg. l(-1)) of dicamba (3,6-dichloro-2metoxibenzoic acid) and picloram (4-amino-3,5,6-trichloropicolinic acid) on the callers induction, direct shoot foramtion rate and callus weight from young inflorescences crested wheatgrass (Agropyron cristatum (L.) Gaertn). The result of the study showed that callus induction rate (`%), shoot formation (%) and callus weight (mg/per Petri dishes) the young inflorescences were signficantly; affected by medium, auxin and auxin concentrations. Depending on the medium, mean callus,induction rate,.shoot formation and callus weight (mg/per Petri dishes) varied from 18% -18.8%, 8.6% - 62.5%, 11.4 - 16.4 mg, respectively Callus induction,.shoot formation rates and callers weight were also significantly influenced by the auxin ripe and auxin concentrations. The highest callus induction rate and callers weight were obtained from the LS medium containing 4 mg. P t(-1) dicamba. The highest direct shoo! formation rate was obtained from the SH medium containing 2 mg. P of picloram..