AFLATOKSİN B1 UYGULANAN RATLARDA METALLOPROTEİNAZ İNHİBİTÖRLERİNİN TEK VE NAC+VİTAMİN E KOMBİNASYONUYLA TEDAVİ VE KORUYUCU ETKİLERİ


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Traş B., Eser H., Özdemir Kütahya Z. , Bahçivan E., Dik B., Bozkurt B., ...Daha Fazla

1st INTERNATIONAL HEALTH SCIENCE AND LIFE CONGRESS, Burdur, Türkiye, 2 - 05 Mayıs 2018, ss.464

  • Basıldığı Şehir: Burdur
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.464

Özet

The aim of study was to determine the effects of single and combined treatments with NAC and vitamin E in different doses of minocycline and dexamethasone on body distribution of AFB1 and changes in LD50 of AFB1 in the treatment presence in rats. The study was carried out on male Wistar rats in two stages. In first stage, after administration of AFB1 (2 mg/kg, IP), minocycline (45 and 90 mg/kg, IP) and dexamethasone (5 and 20 mg/kg, IP) were administered as the single and, combined with NAC (200 mg/kg, IP) and vitamin E (600 mg/kg, IP). Tissue and blood samples were taken from the animals at 12h following AFB1 administration. AFB1 levels were measured using HPLC-FLD. In second stage, the change in LD50 of AFB1 was determined by calculating the therapeutic effect. The administration of dexamethasone at 20 mg/kg versus 5 mg/kg reduced AFB1 levels in plasma and other tissues exist for aqueous humour compared to AFB1 group. Effect of minocycline on plasma and tissue levels of AFB1 varied with the dose and tissue challenge. Combined administration of dexamethasone and minocycline with NAC+vitamin E increased AFB1 levels in all tissues and plasma except aqueous humour and liver. Following administration of dexamethasone at 20 mg/kg, the therapeutic effect value of 1.5 was determined. Dexamethasone can be administered repeatedly at ≥20mg/kg to increase survival in cases of AFB1-related poisoning. Further studies are needed to determine effects of single and repeated doses of >20mg/kg dexamethasone on plasma and tissue levels of AFB1.