Turkey still uses foreign varieties in strawberry growing and pays money for patent rights for each plant. The market requires strawberry varieties with high yield, large fruit, long storage life and having a good appearance and good taste. For these reasons, we aimed to create an F-1 population between cultivar 'Ottoman' (male sterile) and cultivar 'Camarosa' (hermaphrodite), which exhibits many opposite characters to eachother. In addition, we aimed to characterize them with important characters for further genetic studies in strawberry which will provide a transition from classical breeding to a modern one in strawberry. In this study, F-1 seedlings and their parents were characterized based on the fruit yield, fruit weight, fruit firmness, sugar (fructose, glucose and sucrose), acid (malic, citric and ascorbic) contents and flower type in 200 F-1 seedlings.