In Vitro efficacy of cyclosporine a and various antiseptics and antiviral drugs on adenovirus genotype 8, a common cause of epidemic keratoconjunctivitis

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Aydamirov A. S., HARBİYELİ İ. İ., ÖZTÜRK G., YARKIN F., ERDEM E., YAĞMUR M.

INTERNATIONAL OPHTHALMOLOGY, cilt.43, sa.5, ss.1701-1710, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 43 Sayı: 5
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1007/s10792-022-02567-0
  • Dergi Adı: INTERNATIONAL OPHTHALMOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, CAB Abstracts, EMBASE, MEDLINE
  • Sayfa Sayıları: ss.1701-1710
  • Anahtar Kelimeler: Adenovirus genotype 8, Chlorhexidine, Cidofovir, Cyclosporine A, Ganciclovir, Povidone iodine
  • Çukurova Üniversitesi Adresli: Evet

Özet

Purpose To evaluate the in vitro efficacy of cidofovir, ganciclovir, povidone-iodine, chlorhexidine, and cyclosporine A on adenovirus genotype 8. Methods Conjunctival samples were collected from patients with adenoviral conjunctivitis and cultured in A549 cells. Adenovirus diagnosis was confirmed by RT-PCR. For each drug, the 50% cytotoxic concentration (CC 50 ) was determined. Subsequently, the antiviral activity was tested at concentrations below CC 50, and the 50% inhibitor concentration (IC 50 ) of drugs was determined Results While the IC 50 of cidofovir against adenovirus genotype 8 was 3.07 +/- 0.8 mu M, ganciclovir, povidone-iodine, chlorhexidine, and cyclosporine A were not found to be effective against adenovirus genotype 8 at concentrations below the CC 50 value. Conclusions Cidofovir was found effective and the IC 50 value was within the ranges in the literature. Ganciclovir and cyclosporine A were found to be ineffective at doses below the cytotoxic dose, povidone-iodine and chlorhexidine was found to be highly cytotoxic.