Effects of experimental left varicocele repair on hypoxia-inducible factor-1 alpha and vascular endothelial growth factor expressions and angiogenesis in rat testis

Goren M. R., Kilinc F., Kayaselcuk F., Ozer C., Oguzulgen I., Hasirci E.

ANDROLOGIA, cilt.49, sa.2, 2017 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 49 Sayı: 2
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1111/and.12614
  • Dergi Adı: ANDROLOGIA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Anahtar Kelimeler: HIF-1 alpha protein, hypoxia, varicocele, varicocelectomy, vascular endothelial growth factor, SPERM DNA-DAMAGE, MALE-INFERTILITY, PART II, PATHOPHYSIOLOGY, APOPTOSIS, MODEL, INHIBITION
  • Çukurova Üniversitesi Adresli: Evet

Özet

This study was aimed to investigate the effects of experimental left varicocele (ELV) repair on hypoxia-inducible factor-1 alpha (HIF-1 alpha) and vascular endothelial growth factor (VEGF) expressions and angiogenesis in rat testis. ELVs were surgically created in 26 adult male Sprague-Dawley rats. Thirty days after surgery, ELV repair was performed in 13 of the rats. All rats subsequently underwent orchiectomy 30days after the last laparotomy. Histology of ELV-repaired testicles was compared to that of the unrepaired (ELV) group. The frequency of positive HIF-1 alpha findings was significantly lower in the ELV-repaired than in the ELV group. The frequency of positive VEGF findings was also lower in the ELV-repaired than in the ELV group, although the difference was not statistically significant (P = 0.238). The mean microvessel density in ELV-repair group was significantly lower than that in the ELV group (P = 0.002). Our study demonstrated that ELV repair may protect tissues from hypoxia and hypoxia-related pathophysiologic events, such as angiogenesis, in rat testis.